Coding

Part:BBa_K1616022:Design

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-18)


pDawn - Endolysin/Holin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2755
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2689
    Illegal NgoMIV site found at 2787
    Illegal NgoMIV site found at 3670
    Illegal NgoMIV site found at 3742
    Illegal NgoMIV site found at 3832
    Illegal NgoMIV site found at 3850
    Illegal NgoMIV site found at 4344
    Illegal AgeI site found at 306
    Illegal AgeI site found at 376
    Illegal AgeI site found at 3384
    Illegal AgeI site found at 4512
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 3283


Design Notes

The original sequence of pDawn have some illegal sites that have been removed.


Source

This part is composed of Holin/Endolysin toxin BBa_K1616005 and pDawn system BBa_K1616019

References